Once the dilution is made, an aliquot can be spread on an agar plate to create a spread plate. https://www.jove.com/v/10507/serial-dilutions-and-plating-microbial-enumeration, 2. Pipette 5.0 mL of the 5.0 g/mL methylene blue working solution into a 15 mL conical tube. Transfer the sodium acetate into a 50 mL conical tube. These are your samples. Provide a concrete example. Describe some of its practical applications. Use the micropipette to dispense 25 mL of PBS diluent to the first well. What are the concentrations of H3O and OH-? For example, bacterial pathogens can be introduced into foods at any stage: during growth/production at the farm, during processing, during handling and packaging, and when the food is prepared in the kitchen (1). What are the benefits of performing a serial dilution vs. directly diluting varying amounts of a stock solution? (a) What are the principles of chemistry? Choose. Image by Jackie Reynolds, Richland College, Dallas, TX. What is a serial dilution and why might you want to use it? Moreover, it will allow you to get more accurate and precise results, as the pre-wetting steps, pipetting speeds, mixing cycles and mixing volumes will always be consistent. Preventative Maintenance Program for MEDIACLAVE and MEDIAJET, How to do serial dilutions (including calculations), More information on how to calculate the diluent volume, Walk-away pipetting of serial dilutions for MIC testing, Serial dilution in 96 and 384 well plates, https://www.jove.com/v/10507/serial-dilutions-and-plating-microbial-enumeration, https://study.com/academy/lesson/serial-dilution-in-microbiology-calculation-method-technique.html, https://scienceprimer.com/serial-dilution. Subtracting the transfer volume from the final volume needed gives the diluent volume that has to be dispensed into the tubes or wells used to set up a serial dilution: Final volume transfer volume = diluent volume. Describe non-chemical examples of limiting reactants, and when you are comfortable with the concept, relate this concept to any chemical reaction.